Difference between revisions of "Part:BBa K2036022"
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<partinfo>BBa_K2036022 short</partinfo> | <partinfo>BBa_K2036022 short</partinfo> | ||
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This part is pH sensitive on account of pH sensitive promoter patp2. CI and pR are derived from bacteria lmabda operon. CI can block polymerase within pR so that transcription of CIII is inhibited. So it forms a NO gate of CIII expression. | This part is pH sensitive on account of pH sensitive promoter patp2. CI and pR are derived from bacteria lmabda operon. CI can block polymerase within pR so that transcription of CIII is inhibited. So it forms a NO gate of CIII expression. | ||
It serves as an intermediate to construct Prokaryote application circuit of Signal Filter([http://2016.igem.org/Team:HUST-China see HUST-China 2016 project]). This part is built by In-Fusion cloning method and together with [https://parts.igem.org/Part:BBa_K2036021 BBa_K2036021] and [https://parts.igem.org/Part:BBa_K2036023 BBa_K2036023] to further assemble [https://parts.igem.org/Part:BBa_K2036024 BBa_K2036024] | It serves as an intermediate to construct Prokaryote application circuit of Signal Filter([http://2016.igem.org/Team:HUST-China see HUST-China 2016 project]). This part is built by In-Fusion cloning method and together with [https://parts.igem.org/Part:BBa_K2036021 BBa_K2036021] and [https://parts.igem.org/Part:BBa_K2036023 BBa_K2036023] to further assemble [https://parts.igem.org/Part:BBa_K2036024 BBa_K2036024] | ||
| − | [[File:T--HUST-China--Experiments-Fig15.png|thumb| | + | [[File:T--HUST-China--Experiments-Fig15.png|thumb|350px|center|Fig1: Prokaryote application plasmid for lactose intolerance]] |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 08:30, 3 October 2016
patp2-RBS-CI-TT-pR-RBS-CIII
This part is pH sensitive on account of pH sensitive promoter patp2. CI and pR are derived from bacteria lmabda operon. CI can block polymerase within pR so that transcription of CIII is inhibited. So it forms a NO gate of CIII expression. It serves as an intermediate to construct Prokaryote application circuit of Signal Filter([http://2016.igem.org/Team:HUST-China see HUST-China 2016 project]). This part is built by In-Fusion cloning method and together with BBa_K2036021 and BBa_K2036023 to further assemble BBa_K2036024
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]