Difference between revisions of "Part:BBa K1033906"

Revision as of 08:22, 3 October 2016

tsPurple, purple chromoprotein

This chromoprotein (also known as TinselPurple) naturally exhibits strong purple color when expressed.

Usage and Biology

This part is useful as a reporter.

iGEM2013 Uppsala: The images above show E coli constitutively expressing the chromoproteins amilCP BBa_K592009 and tsPurple BBa_K1033906 from the high copy plasmid pSB1C3 from the promoters J23116 and J23110.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Structure and SWISS MODEL Homology Modelling Report

(The following information is the contribution of SVCE_Chennai)

The following sections give a detailed information on this chromoprotein done by its in silico analysis.

3D Structure

Cartoon representation of tsPurple.

QMEAN = 0.02
Cβ = -0.91
All Atom = 0.52
Solvation = -1.19
Torsion = 0.32

Template	Seq. Identity	Oligo-state	Found by	Method	Resolution	Seq Similarity	Range	Coverage	Description
4ohs.1.A	86.61	homotetramer	HHblits	X-Ray	2.19Å	0.58	2 - 222	0.98	FAR-RED FLUORESCENT PROTEIN AQ143

Ligand	Added to Model	Description
CL	✕ - Not biologically relevant.	CHLORIDE ION
CL	✕ - Not biologically relevant.	CHLORIDE ION
CL	✕ - Not biologically relevant.	CHLORIDE ION

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 (The following information is the contribution of SVCE_Chennai)
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 <br>Torsion = 0.32
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