Difference between revisions of "Part:BBa K2092009"
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<partinfo>BBa_K2092009 short</partinfo> | <partinfo>BBa_K2092009 short</partinfo> | ||
| + | The alcA promoter, P<i>alcA</i>, is originally found in <i>Aspergillus nidulans</i> as a part of the ethanol regulon. P<i>alcA</i> requires its positive transcriptional regulator AlcR to regulate the expression of gene <i>alcA</i>. Gene <i>alcA</i> encodes for alcohol dehydrogenase I (ADHI) which facilitates the interconversion between alcohols and carbonyls. This part consists of a P<i>alcA</i> (BBa_K2092002, https://parts.igem.org/Part:BBa_K2092002) variant tagged with a blue chromoprotein, which makes it capable to produce blue colour upon the binding of its ethanol-activated transcription factor AlcR. | ||
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| + | <!-- Add more about the biology of this part here | ||
| + | ===Usage and Biology=== | ||
PalcA is one of the strongest inducible promoters in Aspergillus nidulans commonly used to overexpress proteins [1]. It has been shown that PalcA promoter is also functional in monocotyledonous plant sugar cane [2] and Escherichia coli (E.coli) [3]. Its transcriptional activation is dependent on the binding of its positive transcriptional regulator AlcR with various substrates that employ a hydroxyl group, for example ethanol and threonine. | PalcA is one of the strongest inducible promoters in Aspergillus nidulans commonly used to overexpress proteins [1]. It has been shown that PalcA promoter is also functional in monocotyledonous plant sugar cane [2] and Escherichia coli (E.coli) [3]. Its transcriptional activation is dependent on the binding of its positive transcriptional regulator AlcR with various substrates that employ a hydroxyl group, for example ethanol and threonine. | ||
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This part allows detection of ethanol by coupling it with another composite part, <a href="https://parts.igem.org/Part:BBa_K2092008"> BBa_K2092008</a>, which constitutively expresses its transcription factor, AlcR. Blue color development can be seen in presence of ethanol. | This part allows detection of ethanol by coupling it with another composite part, <a href="https://parts.igem.org/Part:BBa_K2092008"> BBa_K2092008</a>, which constitutively expresses its transcription factor, AlcR. Blue color development can be seen in presence of ethanol. | ||
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Revision as of 23:28, 2 October 2016
PalcA(var) + RBS (B0034) +amilCP The alcA promoter, PalcA, is originally found in Aspergillus nidulans as a part of the ethanol regulon. PalcA requires its positive transcriptional regulator AlcR to regulate the expression of gene alcA. Gene alcA encodes for alcohol dehydrogenase I (ADHI) which facilitates the interconversion between alcohols and carbonyls. This part consists of a PalcA (BBa_K2092002, https://parts.igem.org/Part:BBa_K2092002) variant tagged with a blue chromoprotein, which makes it capable to produce blue colour upon the binding of its ethanol-activated transcription factor AlcR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 274
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]