Difference between revisions of "Part:BBa K2092005"

 
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<partinfo>BBa_K2092005 short</partinfo>
  
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This part encodes for the <i>alcR</i> gene, which is a positive regulatory gene in the ethanol regulon of filamentous fungus <i>Aspergillus nidulans (A.nidulans)</i>. It encodes the AlcR protein, an ethanol-activated transcription factor which would bind to its target promoters, including the alcA <a href="https://parts.igem.org/Part:BBa_K2092002">(PalcA)</a>  and aldA promoters.
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AlcR is also known as a zinc binuclear cluster activator as it contains a DNA-binding domain belonging to the C6 zinc binuclear cluster family. AlcR is unique in that it can bind to symmetric and asymmetric DNA sites with the same apparent affinity and also bind to single site with high affinity [1].
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Reference:
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[1] Felenbok, B., Sequeval, D., Mathieu, M., Sibley, S., Gwynne, D.I. and Davies, R.W. (1988). The ethanol regulon in Aspergillus nidulans: Characterization and sequence of the positive regulatory gene alcR. <i>Gene</i>, 73(2), 385–396.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2092002 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2092002 parameters</partinfo>
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Revision as of 20:19, 2 October 2016

alcR, ethanol-activated transcription factor from A. nidulans (incl. RBS)

This part encodes for the alcR gene, which is a positive regulatory gene in the ethanol regulon of filamentous fungus Aspergillus nidulans (A.nidulans). It encodes the AlcR protein, an ethanol-activated transcription factor which would bind to its target promoters, including the alcA <a href="https://parts.igem.org/Part:BBa_K2092002">(PalcA)</a> and aldA promoters.

AlcR is also known as a zinc binuclear cluster activator as it contains a DNA-binding domain belonging to the C6 zinc binuclear cluster family. AlcR is unique in that it can bind to symmetric and asymmetric DNA sites with the same apparent affinity and also bind to single site with high affinity [1].


Reference:

[1] Felenbok, B., Sequeval, D., Mathieu, M., Sibley, S., Gwynne, D.I. and Davies, R.W. (1988). The ethanol regulon in Aspergillus nidulans: Characterization and sequence of the positive regulatory gene alcR. Gene, 73(2), 385–396.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]