Difference between revisions of "Part:BBa K2042008"
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== Reference: == | == Reference: == | ||
| − | Engineering a d-lactate dehydrogenase that can super-efficiently utilize NADPH and NADH as cofactors. | + | Engineering a d-lactate dehydrogenase that can super-efficiently utilize NADPH and NADH as cofactors.<br> |
| − | Meng H1,2, Liu P3, Sun H2,3, Cai Z2, Zhou J2, Lin J3,4, Li Y2. | + | Meng H1,2, Liu P3, Sun H2,3, Cai Z2, Zhou J2, Lin J3,4, Li Y2.<br> |
| − | + | https://www.ncbi.nlm.nih.gov/pubmed/27109778 | |
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Revision as of 20:00, 1 October 2016
Engineered D-Lactate dehydrogenase (D-LDH*)
This is the coding sequence of D-lactate deshydrogenase from Lactobacillus delbrueckii 11842. The sequence was condon optimized for Pseudomonas putida and point mutations on three amino acids (D176S, I177R, F178T) were found to efficiently use both NADH and NADPH as cofactors. This enzyme would be useful, as the activity of such an enzyme will not be limited by the availability of NADH or NADPH.
Reference:
Engineering a d-lactate dehydrogenase that can super-efficiently utilize NADPH and NADH as cofactors.
Meng H1,2, Liu P3, Sun H2,3, Cai Z2, Zhou J2, Lin J3,4, Li Y2.
https://www.ncbi.nlm.nih.gov/pubmed/27109778
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 630
- 1000COMPATIBLE WITH RFC[1000]