Difference between revisions of "Part:BBa K2092002:Design"
Huiwenchong (Talk | contribs) (→Design Notes) |
Huiwenchong (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
XbaI and SpeI restriction sites were missing in the original BioBrick by 2011 iGEM DTU-Denmark. To add the following sites, two primers were designed: | XbaI and SpeI restriction sites were missing in the original BioBrick by 2011 iGEM DTU-Denmark. To add the following sites, two primers were designed: | ||
| + | |||
alcA forward primer = 5'- CGG AAT TCG CGT CTA GAA CGT CGC TCT CCC CGA TGA C -3' | alcA forward primer = 5'- CGG AAT TCG CGT CTA GAA CGT CGC TCT CCC CGA TGA C -3' | ||
alcA reverse primer = | alcA reverse primer = | ||
Revision as of 16:03, 1 October 2016
PalcA, improved alcR inducible promoter from A. nidulans
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 274
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
XbaI and SpeI restriction sites were missing in the original BioBrick by 2011 iGEM DTU-Denmark. To add the following sites, two primers were designed:
alcA forward primer = 5'- CGG AAT TCG CGT CTA GAA CGT CGC TCT CCC CGA TGA C -3' alcA reverse primer = 5'- AAC TGC AGA AAC CAA TGC ATT GGA CTA GTT TTT GAG GCG AGG TGA TAG GAT TGG -3'
Source
Aspergillus nidulans, sequence found through