Difference between revisions of "Part:BBa K2092002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
The P<i>alcA</i> is one of the strongest inducible promoters in <i>Aspergillus nidulans</i> commonly used to overexpress proteins [1]. It has been shown that alcA promoter is also functional in monocotyledonous plant sugar cane [2] and <i>Escherichia coli</i> [3]. Its transciptional activation is dependent on the binding of its positive transcriptional regulator AlcR with various substrates that employ a hydroxyl group, for example ethanol and threonine. The native P<i>alcA</i> consists of 3 AlcR binding sites. The number and position of the AlcR binding sites on the P<i>alcA</i> are crucial in determining its transcriptional activation strength. It has also been shown that each AlcR target in the P<i>alcA</i> contributes differently to the activation of the downstream protein expression. | The P<i>alcA</i> is one of the strongest inducible promoters in <i>Aspergillus nidulans</i> commonly used to overexpress proteins [1]. It has been shown that alcA promoter is also functional in monocotyledonous plant sugar cane [2] and <i>Escherichia coli</i> [3]. Its transciptional activation is dependent on the binding of its positive transcriptional regulator AlcR with various substrates that employ a hydroxyl group, for example ethanol and threonine. The native P<i>alcA</i> consists of 3 AlcR binding sites. The number and position of the AlcR binding sites on the P<i>alcA</i> are crucial in determining its transcriptional activation strength. It has also been shown that each AlcR target in the P<i>alcA</i> contributes differently to the activation of the downstream protein expression. | ||
Revision as of 16:06, 1 October 2016
PalcA, improved alcR inducible promoter from A. nidulans
The alcA promoter, PalcA, is originally found in Aspergillus nidulans as a part of the ethanol regulon. PalcA requires its positive transcriptional regulator AlcR to regulate the expression of gene alcA. Gene alcA encodes for alcohol dehydrogenase I (ADHI) which facilitates the interconversion between alcohols and carbonyls.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 274
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]