Difference between revisions of "Part:BBa K1739000:Experience"
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<a href="https://parts.igem.org/Part:BBa_K2009357"> BBa_K2009357</a> | <a href="https://parts.igem.org/Part:BBa_K2009357"> BBa_K2009357</a> | ||
| − | </html>.The SUP35NM gene we used is originally provided by Dong Men, PhD of Wuhan Insititue of Virology of Chinese Academy of Sciences, and the sequence is not quite the same as the existing sequence in the Parts Registry, for a lot of mutations have been done. The standardization of this gene is did by ourselves by adding the Biobrick prefix and suffix to its ends and doing a site mutation to eliminate the PstI cutting site inside it. Comparing to the sequence submitted by other team, our SUP35 gene is shorter, which means lower expressing pressure and the possibility to express more protein in the E.coli. | + | </html>.This part's designer is Kaiyue Ma.The SUP35NM gene we used is originally provided by Dong Men, PhD of Wuhan Insititue of Virology of Chinese Academy of Sciences, and the sequence is not quite the same as the existing sequence in the Parts Registry, for a lot of mutations have been done. The standardization of this gene is did by ourselves by adding the Biobrick prefix and suffix to its ends and doing a site mutation to eliminate the PstI cutting site inside it. Comparing to the sequence submitted by other team, our SUP35 gene is shorter, which means lower expressing pressure and the possibility to express more protein in the E.coli. |
Latest revision as of 02:52, 4 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1739000
User Reviews
UNIQa0ae76496f82e101-partinfo-00000000-QINU UNIQa0ae76496f82e101-partinfo-00000001-QINU
Improvement by USTC 2016
Our part is Part:
BBa_K2009357
.This part's designer is Kaiyue Ma.The SUP35NM gene we used is originally provided by Dong Men, PhD of Wuhan Insititue of Virology of Chinese Academy of Sciences, and the sequence is not quite the same as the existing sequence in the Parts Registry, for a lot of mutations have been done. The standardization of this gene is did by ourselves by adding the Biobrick prefix and suffix to its ends and doing a site mutation to eliminate the PstI cutting site inside it. Comparing to the sequence submitted by other team, our SUP35 gene is shorter, which means lower expressing pressure and the possibility to express more protein in the E.coli.