Difference between revisions of "Part:BBa K401001:Experience"
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Our part is Part:<html>
 
Our part is Part:<html>
 
<a href="https://parts.igem.org/Part:BBa_JK2009357"> BBa_K2009357</a>
 
<a href="https://parts.igem.org/Part:BBa_JK2009357"> BBa_K2009357</a>
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</html>.We construted this composite by ligating GAL1 promoter + Kozak sequence (Part:BBa_J63006) and (2) GFP (Part:BBa_E0040). However, the ATG inside the KOZAK sequence won't be in the same reading frame as the ATG of the downstream coding sequence, so a frame-shift mutation is inevitable. To solve this problem, we add a base pair after the Kozak sequence so that the ATG can be in the same reading frame and the GFP can be expressed properly. What’s more, this part become “ready to use”, which means the GFP sequence can be directly altered by other functional parts and the sequence will be expressed properly.
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</html>.The SUP35NM gene we used is originally provided by Dong Men, PhD of Wuhan Insititue of Virology of Chinese Academy of Sciences, and the sequence is not quite the same as the existing sequence in the Parts Registry, for a lot of mutations have been done. The standardization of this gene is did by ourselves by adding the Biobrick prefix and suffix to its ends and doing a site mutation to eliminate the PstI cutting site inside it. Comparing to the sequence submitted by other team, our SUP35 gene is shorter, which means lower expressing pressure and the possibility to express more protein in the E.coli.

Revision as of 03:15, 1 October 2016


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Please enter how you used this part and how it worked out.

Applications of BBa_K401001

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UNIQe635fd13b4044156-partinfo-00000000-QINU UNIQe635fd13b4044156-partinfo-00000001-QINU

Improvement by USTC 2016


Our part is Part: BBa_K2009357 .The SUP35NM gene we used is originally provided by Dong Men, PhD of Wuhan Insititue of Virology of Chinese Academy of Sciences, and the sequence is not quite the same as the existing sequence in the Parts Registry, for a lot of mutations have been done. The standardization of this gene is did by ourselves by adding the Biobrick prefix and suffix to its ends and doing a site mutation to eliminate the PstI cutting site inside it. Comparing to the sequence submitted by other team, our SUP35 gene is shorter, which means lower expressing pressure and the possibility to express more protein in the E.coli.