Difference between revisions of "Part:BBa K2036018"
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| − | pRE-RBS-Cro-RBS-CII-TT | + | This is a part of Prokaryote version of Signal Filter characterization circuit:pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K2036027 BBa_K2036027]). |
| − | + | [[File:T--HUST-China--Experiments-Fig14-1.png|thumb|500px|center|Fig1:Prokaryote version of Signal Filter characterization circuit]] | |
| − | + | <br>Because the circuit is too complex we divided it into three parts and each is assembled by In-Fusion method (homologous recombination). The whole circuit built by 3A assembly and then we transfer the backbone from pSB1C3 to PET-Duet-1. | |
| − | + | [[File:T--HUST-China--Experiments-Fig13.png|thumb|500px|center|Fig2:Prokaryote version of Signal Filter characterization plasmid]] | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 06:31, 29 September 2016
pRE-RBS-Cro-RBS-CII-TT-ptrp2
This is a part of Prokaryote version of Signal Filter characterization circuit:pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag (BBa_K2036027).
Because the circuit is too complex we divided it into three parts and each is assembled by In-Fusion method (homologous recombination). The whole circuit built by 3A assembly and then we transfer the backbone from pSB1C3 to PET-Duet-1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]