Difference between revisions of "Part:BBa K1895004"

Revision as of 10:20, 28 September 2016

Porin Overexpression for Microbial Fuel Cell Use

As part of our iGEM project we worked on trying to improve the current output from a microbial fuel cell. Microbial fuel cells which use E. coli depend on a mediator, usually methylene blue to transfer electrons between the electrodes and the organisms electron transport chain. The mediator enters the cell through porins in the cell membrane and 'steals' electrons, becoming oxidised. These electrons are then carried to the cathode, which is positively charged where they are lost, allowing electrons to flow around the circuit.

By increasing the amount of mediator that can enter the cell we hope to be able to increase the electron flow rate, or current, from our microbial fuel cell. This occurs because the electrons can be moved more freely and they're movement via the mediator is less limited by the rate at which the cell can transport the mediator across the membrane.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 255
Illegal BamHI site found at 65
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 388
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1895004 short</partinfo>
-[TODO]
+As part of our iGEM project we worked on trying to improve the current output from a microbial fuel cell. Microbial fuel cells which use E. coli depend on a mediator, usually methylene blue to transfer electrons between the electrodes and the organisms electron transport chain. The mediator enters the cell through porins in the cell membrane and 'steals' electrons, becoming oxidised. These electrons are then carried to the cathode, which is positively charged where they are lost, allowing electrons to flow around the circuit.
+By increasing the amount of mediator that can enter the cell we hope to be able to increase the electron flow rate, or current, from our microbial fuel cell. This occurs because the electrons can be moved more freely and they're movement via the mediator is less limited by the rate at which the cell can transport the mediator across the membrane.
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