Difference between revisions of "Part:BBa K2036005"

Revision as of 02:13, 3 October 2016

PP2CA,a type of protein from subfamily of type 2C protein phosphatases

Abscisic acid (ABA) is an essential hormone for plants to survive environmental stresses. As one of important part in ABA signaling network , PP2CA can form exclusive interactions with ABA receptors and subfamily 2 Snfl-related kinase. Also it can directly dephosphorylate the ABFs transcription factors. These two functions both lead to inactivation of the Snf1-related(SnRK)2 clade of protein kinases, and inactivation of their downstream targets such as ABA-response element binding basic leucine zipper (bZIP) transcription factors.
Application in project: we apply PP2CA into our eukaryotic filter .When pulse OFF appears,PP2CA can dephosphorylate ABF2 and inhibite the function of SnRK2.2,these both block the expression of genes downstream the RD29A promoter.

Fig1:Eukaryote version of Signal Filter

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 90
Illegal BamHI site found at 661
Illegal BamHI site found at 806
Illegal BamHI site found at 918
Illegal XhoI site found at 65
Illegal XhoI site found at 875
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 48
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2036005 short</partinfo>
-Abscisic acid (ABA) is an essential hormone for plants to survive environmental stresses. As one of important part in ABA signaling network , PP2CA can form exclusive interactions with ABA receptors and subfamily 2 Snfl-related kinase. Also it can directly dephosphorylate the ABFs transcription facrors. These two functions both lead to inactivation of the Snf1-related(SnRK)2 clade of protein kinases, and inactivation of their downstream targets such as ABA-response element binding basic leucine zipper (bZIP) transcription factors.
+Abscisic acid (ABA) is an essential hormone for plants to survive environmental stresses. As one of important part in ABA signaling network , PP2CA can form exclusive interactions with ABA receptors and subfamily 2 Snfl-related kinase. Also it can directly dephosphorylate the ABFs transcription factors. These two functions both lead to inactivation of the Snf1-related(SnRK)2 clade of protein kinases, and inactivation of their downstream targets such as ABA-response element binding basic leucine zipper (bZIP) transcription factors.
 <br>Application in project: we apply PP2CA into our eukaryotic filter .When pulse OFF appears,PP2CA can dephosphorylate ABF2 and inhibite the function of SnRK2.2,these both block the expression of genes downstream the RD29A promoter.
 [[File:T--HUST-China--Description-Fig-prokaryote.png|thumb|500px|center|Fig1:Eukaryote version of Signal Filter]]