Difference between revisions of "Part:BBa K2100002:Experience"

 
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===Applications of BBa_K2100002===
 
===Applications of BBa_K2100002===
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Experiment in tHESC:
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We transfected tHESC cells with 250ng of hEF1a-mKate as a transfection marker and 250 ng pERE5-eYFP to examine the promoter's transcriptional activity by observing increases in yellow fluorescence upon cells being induced with 5 nM E2.
 +
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The results show a fold difference in yellow fluorescent output between the induced MCF-7 cells and the uninduced cells with a vehicle control (0.4% EtOH) to account for the possible increase in proliferation cells undergo when being induced with E2 dissolved in ethanol.
 +
 +
Experiment in tHESC:
 +
 +
We transfected tHESC cells with 250ng of hEF1a-mKate as a transfection marker and 250 ng pERE5-eYFP to examine the promoter's transcriptional activity by observing increases in yellow fluorescence upon cells being induced with 5 nM E2.
 +
 +
The results show a fold difference in yellow fluorescent output between the induced MCF-7 cells and the uninduced cells with a vehicle control (0.4% EtOH) to account for the possible increase in proliferation cells undergo when being induced with E2 dissolved in ethanol.
 +
 +
Experiment in tHESC:
 +
 +
We transfected tHESC cells with 250ng of hEF1a-mKate as a transfection marker and 250 ng pERE5-eYFP to examine the promoter's transcriptional activity by observing increases in yellow fluorescence upon cells being induced with 5 nM E2.
 +
 +
The results show a fold difference in yellow fluorescent output between the induced MCF-7 cells and the uninduced cells with a vehicle control (0.4% EtOH) to account for the possible increase in proliferation cells undergo when being induced with E2 dissolved in ethanol.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 23:11, 17 October 2016


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Please enter how you used this part and how it worked out.

Applications of BBa_K2100002

Experiment in tHESC:

We transfected tHESC cells with 250ng of hEF1a-mKate as a transfection marker and 250 ng pERE5-eYFP to examine the promoter's transcriptional activity by observing increases in yellow fluorescence upon cells being induced with 5 nM E2.

The results show a fold difference in yellow fluorescent output between the induced MCF-7 cells and the uninduced cells with a vehicle control (0.4% EtOH) to account for the possible increase in proliferation cells undergo when being induced with E2 dissolved in ethanol.

Experiment in tHESC:

We transfected tHESC cells with 250ng of hEF1a-mKate as a transfection marker and 250 ng pERE5-eYFP to examine the promoter's transcriptional activity by observing increases in yellow fluorescence upon cells being induced with 5 nM E2.

The results show a fold difference in yellow fluorescent output between the induced MCF-7 cells and the uninduced cells with a vehicle control (0.4% EtOH) to account for the possible increase in proliferation cells undergo when being induced with E2 dissolved in ethanol.

Experiment in tHESC:

We transfected tHESC cells with 250ng of hEF1a-mKate as a transfection marker and 250 ng pERE5-eYFP to examine the promoter's transcriptional activity by observing increases in yellow fluorescence upon cells being induced with 5 nM E2.

The results show a fold difference in yellow fluorescent output between the induced MCF-7 cells and the uninduced cells with a vehicle control (0.4% EtOH) to account for the possible increase in proliferation cells undergo when being induced with E2 dissolved in ethanol.

User Reviews

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