Difference between revisions of "Part:BBa K2036030"

Revision as of 09:21, 3 October 2016

PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc-Kozak-ABF2-TTADH1-prd29A-Kozak-GFP-LVAssrAtag

ABF2 can be phosphorylated by Snrk2.2 to activate promoter RD29A and thus starting the expression of the target gene and also forming a positive feedback system. ABF2 can be dephosphorylated by PP2CA to stop the whole process, making the bi-stable state of our circuit.

Fig1:bi-stable function of Signal Filter Eukaryotic circuit

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 3756
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 3756
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 90
Illegal BglII site found at 3824
Illegal BamHI site found at 661
Illegal BamHI site found at 806
Illegal BamHI site found at 918
Illegal BamHI site found at 1816
Illegal BamHI site found at 2473
Illegal XhoI site found at 65
Illegal XhoI site found at 875
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 3756
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 3756
Illegal AgeI site found at 48
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 5268
Illegal BsaI.rc site found at 5939

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2036030 short</partinfo>
 ABF2 can be phosphorylated by Snrk2.2 to activate promoter RD29A and thus starting the expression of the target gene and also forming a positive feedback system. ABF2 can be dephosphorylated by PP2CA to stop the whole process, making the bi-stable state of our circuit.
+[[File:T--HUST-China--Description-Fig-prokaryote.png|thumb|400px|center|Fig1:bi-stable function of Signal Filter Eukaryotic circuit]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===