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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K1921001 SequenceAndFeatures</partinfo>
 
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===Functional Parameters===
 
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Revision as of 08:03, 25 September 2016

MHETase


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1027
    Illegal AgeI site found at 247
    Illegal AgeI site found at 364
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage

PETase is dedicated to the role of PET degradation. Our subject of the competition for this year is to modify PETase and developing cell surface display.

Biology

PETase was found from a kind of microorganism（Ideonella sakaiensis 201-F6） living on PET as the main carbon source. It can degrade macromolecular polymers into monomers.PETase is the only enzyme found in bacteria which can degrade PET. Compare to the other enzyme found in fungi like LCC, TfH, FsC, PETase is much more active under low temperature environment, which means its reaction conditions is feasible in practical application than the others'.Additionally, PETase has been shown to have a degrading efficiency 120 times greater than alternative enzymes.

Reference