Difference between revisions of "Part:BBa K2036027"

 
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<partinfo>BBa_K2036027 short</partinfo>
 
<partinfo>BBa_K2036027 short</partinfo>
  
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pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag
+
Cro represses pRM.CII can be rapidly degraded by FtsH.CIII acts as an inhibitor of FtsH.PRE promoter of bacteriophage λ is active only in the presence of CII protein.
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Revision as of 09:07, 25 September 2016


pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag

Cro represses pRM.CII can be rapidly degraded by FtsH.CIII acts as an inhibitor of FtsH.PRE promoter of bacteriophage λ is active only in the presence of CII protein.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 717
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 717
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 717
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 717
    Illegal AgeI site found at 2533
    Illegal AgeI site found at 2645
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3561