Difference between revisions of "Part:BBa K2060001:Design"
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| + | <h3>Design</h3> | ||
| + | <p>The mKeima sequence was taken from [https://www.ncbi.nlm.nih.gov/protein/AMO27260.1 GenBank] and was synthesised as an [http://www.idtdna.com/pages/products/genes/gblocks-gene-fragments IDT gBlock] downstream of the well-characterised pBAD Arabinose inducible promotor and a RBS (tcacacaagaagg) together with the Biobrick Prefix and Suffix. This gBlock was 878bp in total.</p> | ||
| + | <p>We cloned the gBlock into pSB1C3 by restriction digestion with EcoRI and PstI.</p> | ||
| + | |||
| + | <p>We confirmed the entire construct by sanger sequencing</p> | ||
| + | |||
| + | http://2016.igem.org/File:T--Cardiff_Wales--mKeimaSeq.png | ||
Revision as of 10:57, 19 October 2016
Design
The mKeima sequence was taken from GenBank and was synthesised as an [http://www.idtdna.com/pages/products/genes/gblocks-gene-fragments IDT gBlock] downstream of the well-characterised pBAD Arabinose inducible promotor and a RBS (tcacacaagaagg) together with the Biobrick Prefix and Suffix. This gBlock was 878bp in total.
We cloned the gBlock into pSB1C3 by restriction digestion with EcoRI and PstI.
We confirmed the entire construct by sanger sequencing
http://2016.igem.org/File:T--Cardiff_Wales--mKeimaSeq.png