Difference between revisions of "Part:BBa K1555000:Experience"

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<I>HSiTAIWAN</I>
 
<I>HSiTAIWAN</I>
 
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This part works great! HSiTAIWAN iGEM team 2016 used this part to detect copper ions in Chinese Medicine. The results were as expected. The reporter performed well without being interfered by complex substances. This part performed in an expected pattern and is an useful reporter.
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        <h2><strong>Detecting Copper in Chinese medicine</strong></h2>
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<h4>Objective</h4>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Measure the E. coli fluorescence intensity in different copper ion concentration with 3μg/ml Chinese medicine.</p>
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<h4>Methods</h4>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Copper ion solution with different copper ion concentration (0, 3, 9, 27ppm) is added into E. coli DH5α with Plasmids encoding GFP under copper promoter(part: BBa_K1555000).<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Fluorescence was measured at: <br>
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<ul>
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<li><em>Excitation peak: 470nm</em></li>
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<li><em>Emission peak: 508nm</em></li>
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</ul>
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every 30 minutes.
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<h4>Results</h4>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This part is still specific to copper in Chinese medicine, so it be apply in Chinese medicine examination.
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                <center>
 
https://static.igem.org/mediawiki/parts/6/66/2016_hsitaiwan_cu_1.png
 
https://static.igem.org/mediawiki/parts/6/66/2016_hsitaiwan_cu_1.png
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                </center>
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<h4>Discussion</h4>
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        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Since E. coli expresses stronger fluorescence in higher copper concentration, this is a relevant promoter to detect the existence and measure the amount of arsenic.
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This part works great! HSiTAIWAN iGEM team 2016 used this part to detect copper ions in Chinese Medicine. The results were as expected. The reporter performed well without being interfered by complex substances. This part performed in an expected pattern and is an useful reporter.
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Revision as of 11:50, 23 September 2016

This sensor can be activated in low copper concentration and it's truely useful.

2h 0mg.jpg 2h 0mg/L copper ion 2h 1mg.jpg 2h 1mg/L copper ion

Copa.png

Mcopa.png

Applications of BBa_K1555000

User Reviews

UNIQ9dd53715c9a30b3f-partinfo-00000000-QINU

•••••

HSiTAIWAN

Detecting Copper in Chinese medicine

Objective

     Measure the E. coli fluorescence intensity in different copper ion concentration with 3μg/ml Chinese medicine.

Methods

     Copper ion solution with different copper ion concentration (0, 3, 9, 27ppm) is added into E. coli DH5α with Plasmids encoding GFP under copper promoter(part: BBa_K1555000).
     Fluorescence was measured at:

  • Excitation peak: 470nm
  • Emission peak: 508nm

every 30 minutes.

Results

     This part is still specific to copper in Chinese medicine, so it be apply in Chinese medicine examination.

2016_hsitaiwan_cu_1.png

Discussion

     Since E. coli expresses stronger fluorescence in higher copper concentration, this is a relevant promoter to detect the existence and measure the amount of arsenic.

;

This part works great! HSiTAIWAN iGEM team 2016 used this part to detect copper ions in Chinese Medicine. The results were as expected. The reporter performed well without being interfered by complex substances. This part performed in an expected pattern and is an useful reporter. |width='80%' valign='top'|


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UNIQ9dd53715c9a30b3f-partinfo-00000002-QINU