Difference between revisions of "Part:BBa K2010000"
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This sequence is the E. coli K12 optimized DNA sequence for PETase.
 
This sequence is the E. coli K12 optimized DNA sequence for PETase.
  
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This part includes a promoter, RBS, and PETase fused with a His-tag, for purification.
  
 
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<!-- Add more about the biology of this part here

Revision as of 19:17, 2 October 2016


PETase (PET-degrading enzyme, origin I. sakaiensis)

PETase is the PET(poly(ethylene terephtalate))-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.

This part includes a promoter, RBS, and PETase fused with a His-tag, for purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 426