Difference between revisions of "Part:BBa J100305:Experience"
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| − | We grew E. coli cells with three different experimental groups; negative control should be green, positive control should be red, and UPP promoter. We took a colony from each experimental group, with two colonies from UPP, and grew one of each with UTP, which should have activated the promoter. This graph contains the averages of 3 samples from each experimental group with and without UTP. We measured the fluorescence and absorbance of each sample using a spectrophotometer and calculated the relative fluorescence intensity by finding the ratio between the fluorescence and absorbance (cell density). | + | We grew <i>E. coli </i>cells with three different experimental groups; negative control should be green, positive control should be red, and UPP promoter. We took a colony from each experimental group, with two colonies from UPP, and grew one of each with UTP, which should have activated the promoter. This graph contains the averages of 3 samples from each experimental group with and without UTP. We measured the fluorescence and absorbance of each sample using a spectrophotometer and calculated the relative fluorescence intensity by finding the ratio between the fluorescence and absorbance (cell density). |
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Latest revision as of 19:27, 22 September 2016
We grew E. coli cells with three different experimental groups; negative control should be green, positive control should be red, and UPP promoter. We took a colony from each experimental group, with two colonies from UPP, and grew one of each with UTP, which should have activated the promoter. This graph contains the averages of 3 samples from each experimental group with and without UTP. We measured the fluorescence and absorbance of each sample using a spectrophotometer and calculated the relative fluorescence intensity by finding the ratio between the fluorescence and absorbance (cell density).
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