Difference between revisions of "Part:BBa J100305:Experience"

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describe how you did this
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We grew E. coli cells with three different experimental groups; negative control should be green, positive control should be red, and UPP promoter. We took a colony from each experimental group, with two colonies from UPP, and grew one of each with UTP, which should have activated the promoter. This graph contains the averages of 3 samples from each experimental group with and without UTP. We measured the fluorescence and absorbance of each sample using a spectrophotometer and calculated the relative fluorescence intensity by finding the ratio between the fluorescence and absorbance (cell density).
  
 
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Revision as of 19:26, 22 September 2016


We grew E. coli cells with three different experimental groups; negative control should be green, positive control should be red, and UPP promoter. We took a colony from each experimental group, with two colonies from UPP, and grew one of each with UTP, which should have activated the promoter. This graph contains the averages of 3 samples from each experimental group with and without UTP. We measured the fluorescence and absorbance of each sample using a spectrophotometer and calculated the relative fluorescence intensity by finding the ratio between the fluorescence and absorbance (cell density).

UPP RFP.png


UPP GFP.png


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