Difference between revisions of "Part:BBa K1994003:Experience"

 
 
Line 5: Line 5:
  
 
===Applications of BBa_K1994003===
 
===Applications of BBa_K1994003===
 +
 +
This part was characterised by the Warwick iGEM 2016 team.
 +
 +
This superfolder GFP was incorporated into a larger construct including a weakened omega promoter and dCas9 as a secondary gene. Three versions of this construct were tested using a growth and fluorescence assay, measuring OD600, and emission of GFP at 530nm. This data demonstrates the difference in fluorescence expression between constructs containing the weak omega promoter and a PheA terminator, containing only the terminator, and constructs containing neither.
 +
 +
<br>
 +
<br>
 +
 +
https://static.igem.org/mediawiki/2016/c/c7/OD600ComparisonGFP-dCas9Versions.png
 +
 +
<br>
 +
 +
https://static.igem.org/mediawiki/2016/1/17/GFPComparisonPromoterPositive.png
 +
 +
<br>
 +
 +
https://static.igem.org/mediawiki/2016/3/3e/GFPComparisonPheAPositive.png
 +
 +
<br>
 +
<br>
 +
 +
The graphs show that expression of GFP is much higher in cells containing the weak omega promoter. There is also negligible difference in growth between cells containing the plasmid with the promoter and without.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 23:16, 19 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1994003

This part was characterised by the Warwick iGEM 2016 team.

This superfolder GFP was incorporated into a larger construct including a weakened omega promoter and dCas9 as a secondary gene. Three versions of this construct were tested using a growth and fluorescence assay, measuring OD600, and emission of GFP at 530nm. This data demonstrates the difference in fluorescence expression between constructs containing the weak omega promoter and a PheA terminator, containing only the terminator, and constructs containing neither.



OD600ComparisonGFP-dCas9Versions.png


GFPComparisonPromoterPositive.png


GFPComparisonPheAPositive.png



The graphs show that expression of GFP is much higher in cells containing the weak omega promoter. There is also negligible difference in growth between cells containing the plasmid with the promoter and without.

User Reviews

UNIQ7828f207af3ae027-partinfo-00000000-QINU UNIQ7828f207af3ae027-partinfo-00000001-QINU