Difference between revisions of "Part:BBa K1895000"

Revision as of 19:44, 18 October 2016

Electrically induced promoter system v1

This is a composite part comprising the heat shock promoter BBa_J4450 from MIT 2006, which drives expression of a two-gene operon consisting of a rpoH sigma 32 transcription factor followed by superfolder GFP.

This part was designed for Newcastle iGEM 2016's Culture Shock project in order to induce expression of a downstream gene upon exposure to an electric current. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 738
Illegal SapI.rc site found at 1087
Illegal SapI.rc site found at 1335

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1895000 short</partinfo>
-This is a composite part comprising the heat shock promoter BBa XXX from MIT 2006, which drives expression of a two-gene operon consisting of a rpoH sigma 32 transcription factor followed by superfolder GFP.
+This is a composite part comprising the heat shock promoter BBa_J4450 from MIT 2006, which drives expression of a two-gene operon consisting of a rpoH sigma 32 transcription factor followed by superfolder GFP.
 This part was designed for Newcastle iGEM 2016's Culture Shock project in order to induce expression of a downstream gene upon exposure to an electric current.