Difference between revisions of "Part:BBa K1106004:Experience"

Revision as of 12:22, 20 September 2016

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Applications of BBa_K1106004

User Reviews

UNIQe1b1869597916645-partinfo-00000000-QINU

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HSiTAIWAN

Objective

Measure the E. coli fluorescence intensity in different arsenic concentration with 3μg/ml Chinese medicine.

Methods

Arsenic solution with different arsenic acid concentration (0, 1, 10, 50 and 100 ppm) is added into E. coli DH5α with Plasmids encoding GFP under arsenic promoter(part: BBa_K1106004).
Fluorescence was measured at:

Excitation peak: 485nm
Emission peak: 538nm
Auto cutoff: 515nm

every 30 minutes.

Results

E. coli expresses stronger fluorescence in higher arsenic concentration.

Discussion

This is a relevant promoter to detect the existence and measure the amount of arsenic.

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UNIQe1b1869597916645-partinfo-00000002-QINU

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Igem BsAs 2013

Objective Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concrentrations.

Methods Several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm).

Results As shown in the graphics below GFP production increases with the arsenite concentration.

Conclusion The promoter is sensitive to different arsenite concentrations.

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-	        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This is a relevant promoter to detect and measure the amount of arsenic.
+	        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This is a relevant promoter to detect the existence and measure the amount of arsenic.
 	|};
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