Difference between revisions of "Part:BBa K2036015"

 
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<partinfo>BBa_K2036015 short</partinfo>
 
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CII acts as a necessary and sufficient cis-acting target for rapid proteolysis by initiating pRE. The circuit is built to test CII and pRE interaction together with RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K2036013 BBa_K2036013]) and pRE-RBS-GFP-LVAssrAtag ( [https://parts.igem.org/Part:BBa_K2036011 BBa_K2036011]) . Considering Ftsh protease degrades it raidly in the host, RBS-CII-RBS-CII tandom expression circuit is constructed.
  
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RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAtag
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[[File:T--HUST-China--Experiments-Fig10.png |thumb|500px|center|Fig1:CII and pRE interaction characterization circuits]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 05:10, 29 September 2016


RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag CII acts as a necessary and sufficient cis-acting target for rapid proteolysis by initiating pRE. The circuit is built to test CII and pRE interaction together with RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036013) and pRE-RBS-GFP-LVAssrAtag ( BBa_K2036011) . Considering Ftsh protease degrades it raidly in the host, RBS-CII-RBS-CII tandom expression circuit is constructed.

Fig1:CII and pRE interaction characterization circuits

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1770