Difference between revisions of "Part:BBa K2019001"
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<partinfo>BBa_K2019001 short</partinfo>
 
<partinfo>BBa_K2019001 short</partinfo>
  
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Part BBa_K2019000 expresses crispr associated protein 9 extracted from staphylococcus aureus (saCas9) in E. Coli.  saCas9 is much smaller than the typical cas9 extracted form the genome of streptococcus thermophilus (spCas9), as it the gene that encodes for saCas9 is ~1000bp shorter than that of spCas9.
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Part BBa_K2019000 expresses crispr associated protein 9 extracted from staphylococcus aureus (saCas9) in E. Coli.  saCas9 is much smaller than the typical cas9 extracted form the genome of streptococcus thermophilus (spCas9), as the gene that encodes for saCas9 is ~1000bp shorter than that of spCas9.
  
  

Revision as of 18:25, 15 September 2016


mRFP+gRNA Template (for saCas9)

Part BBa_K2019000 expresses crispr associated protein 9 extracted from staphylococcus aureus (saCas9) in E. Coli. saCas9 is much smaller than the typical cas9 extracted form the genome of streptococcus thermophilus (spCas9), as the gene that encodes for saCas9 is ~1000bp shorter than that of spCas9.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1099
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1076
    Illegal SpeI site found at 1099
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1099
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1099
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1154
    Illegal BsaI.rc site found at 1106