Difference between revisions of "Part:BBa J100301"
| Line 5: | Line 5: | ||
The promoter ompW is regulated by the inhibitor protein ArcA. In the presence of 4mM hydrogen peroxide, ArcA is activated and, therefore, ompW is inhibited. For our project, we will use a 56-base pair long sequence of DNA that we will place into a receiving plasmid using BsaI sticky ends. We will then use bacterial transformation to determine the functionality of our promoter sequence. | The promoter ompW is regulated by the inhibitor protein ArcA. In the presence of 4mM hydrogen peroxide, ArcA is activated and, therefore, ompW is inhibited. For our project, we will use a 56-base pair long sequence of DNA that we will place into a receiving plasmid using BsaI sticky ends. We will then use bacterial transformation to determine the functionality of our promoter sequence. | ||
| − | < | + | <h1>Biology</h1> |
| − | + | <p>Anaerobiosis, is a predominant physiological adaptation and it is mediated by the Aerobic respiratory control B (ArcAB). | |
| + | The two-component system ArcA/B senses the redox status in the cytoplasmic membrane of bacteria through its membrane sensor ArcB, and upon activated, primarily represses the expression of genes involved in the aerobic carbon oxidation through its cognate response regulator ArcA which is phosphorylated under this conditions and its active. | ||
| + | Furthermore, bacterium as Salmonella enterica must sense H2O2 and modulate gene expression. ArcA/B system plays a role in the resistance to reactive oxygen species, under high H202 concentrations ArcA is activated and some targets, as ompW promoter, are negatively regulated. </p> | ||
<!-- --> | <!-- --> | ||
Revision as of 02:58, 22 October 2019
ompW Promoter
The promoter ompW is regulated by the inhibitor protein ArcA. In the presence of 4mM hydrogen peroxide, ArcA is activated and, therefore, ompW is inhibited. For our project, we will use a 56-base pair long sequence of DNA that we will place into a receiving plasmid using BsaI sticky ends. We will then use bacterial transformation to determine the functionality of our promoter sequence.
Biology
Anaerobiosis, is a predominant physiological adaptation and it is mediated by the Aerobic respiratory control B (ArcAB). The two-component system ArcA/B senses the redox status in the cytoplasmic membrane of bacteria through its membrane sensor ArcB, and upon activated, primarily represses the expression of genes involved in the aerobic carbon oxidation through its cognate response regulator ArcA which is phosphorylated under this conditions and its active. Furthermore, bacterium as Salmonella enterica must sense H2O2 and modulate gene expression. ArcA/B system plays a role in the resistance to reactive oxygen species, under high H202 concentrations ArcA is activated and some targets, as ompW promoter, are negatively regulated.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]