Difference between revisions of "Part:BBa K2100000:Experience"

 
Line 5: Line 5:
  
 
===Applications of BBa_K2100000===
 
===Applications of BBa_K2100000===
 +
 +
We characterized the synthetic ERE3 promoter in three cell lines: MCF-7, tHESC, and ISH. All cell lines have endogeneous Estrogen Receptor alpha. We analyzed data from cells induced with estradiol (E2) and uninduced as a control.
 +
 +
 +
Experiment in tHESC:
 +
 +
We transfected tHESC cells with 250ng of hEF1a-mKate as a transfection marker and pERE3-eYFP to examine the promoter's transcriptional activity by observing increases in yellow fluorescence upon cells being induced with 50 nM E2.
 +
 +
http://2016.igem.org/File:T--MIT--bhandarkar_ere3thesc.png
 +
 +
The results show a 9 fold difference in yellow fluorescent output between the induced tHESC cells and the uninduced tHESC cells with a vehicle control (0.4% EtOH) to account for the possible increase in proliferation cells undergo when being induced with E2 dissolved in ethanol.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 22:52, 17 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2100000

We characterized the synthetic ERE3 promoter in three cell lines: MCF-7, tHESC, and ISH. All cell lines have endogeneous Estrogen Receptor alpha. We analyzed data from cells induced with estradiol (E2) and uninduced as a control.


Experiment in tHESC:

We transfected tHESC cells with 250ng of hEF1a-mKate as a transfection marker and pERE3-eYFP to examine the promoter's transcriptional activity by observing increases in yellow fluorescence upon cells being induced with 50 nM E2.

http://2016.igem.org/File:T--MIT--bhandarkar_ere3thesc.png

The results show a 9 fold difference in yellow fluorescent output between the induced tHESC cells and the uninduced tHESC cells with a vehicle control (0.4% EtOH) to account for the possible increase in proliferation cells undergo when being induced with E2 dissolved in ethanol.

User Reviews

UNIQcdccc4229109e727-partinfo-00000000-QINU UNIQcdccc4229109e727-partinfo-00000001-QINU