Difference between revisions of "Part:BBa K2027008"

 
 
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<partinfo>BBa_K2027008 short</partinfo>
 
<partinfo>BBa_K2027008 short</partinfo>
  
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To upregulate L-tyrosine production, we investigated and biobricked a feedback resistant variant of the DAHP synthase isoenzymes AroF (BBa_K2027008). AroF is a species similar to AroG, recommended by Dr. Guillermo Gosset of La Universidad Nacional Autónoma de México. This part was codon-optimized for <i>E. coli</i> and synthesized by IDT.
  
 
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Latest revision as of 07:52, 27 October 2016


Feedback Resistant DAHP Synthase

To upregulate L-tyrosine production, we investigated and biobricked a feedback resistant variant of the DAHP synthase isoenzymes AroF (BBa_K2027008). AroF is a species similar to AroG, recommended by Dr. Guillermo Gosset of La Universidad Nacional Autónoma de México. This part was codon-optimized for E. coli and synthesized by IDT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 499
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25