Difference between revisions of "Part:BBa K1919000:Design"

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===Source===
 
===Source===
  
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The original sequence of CecropinXJ comes from GenBank NM_001043995.1.
+
The original sequence of CecropinXJ comes from GenBank NM_001043995.1. The sample used for experiments was artificially synthesized by Tsingke.
  
 
===References===
 
===References===
 
Xia L, Zhang F, Liu Z, et al. Expression and characterization of cecropinXJ, a bioactive antimicrobial peptide from Bombyx mori (Bombycidae, Lepidoptera) in Escherichia coli[J]. Experimental & Therapeutic Medicine, 2013, 5(6):1745-1751.
 
Xia L, Zhang F, Liu Z, et al. Expression and characterization of cecropinXJ, a bioactive antimicrobial peptide from Bombyx mori (Bombycidae, Lepidoptera) in Escherichia coli[J]. Experimental & Therapeutic Medicine, 2013, 5(6):1745-1751.

Latest revision as of 13:04, 7 September 2016


The coding sequence of antimicrobial peptide CecropinXJ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence was optimized for E.coli expression using JCat http://www.jcat.de/. The single stop codon was replaced by double TAA stop codon.


Source

The original sequence of CecropinXJ comes from GenBank NM_001043995.1. The sample used for experiments was artificially synthesized by Tsingke.

References

Xia L, Zhang F, Liu Z, et al. Expression and characterization of cecropinXJ, a bioactive antimicrobial peptide from Bombyx mori (Bombycidae, Lepidoptera) in Escherichia coli[J]. Experimental & Therapeutic Medicine, 2013, 5(6):1745-1751.