Difference between revisions of "Part:BBa K1943009:Design"

 
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===Design Notes===
 
===Design Notes===
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In our lab, we usually use sfGFP as a report for molecular experiment. In the plasmid, there is a sfGFP coding device which contains promoter BBa_J23116 and RBS BBa_B0034. We design primer and do PCR of this sfGFP coding device and ligate it to BBa_B0015 (adding a terminator) that we do double enzyme digestion, EcoRI & XbaI.
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===Source===
 
===Source===

Revision as of 13:48, 16 October 2016


sfGFP+B0015, green fluorescence protein reporter system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 74


Design Notes

In our lab, we usually use sfGFP as a report for molecular experiment. In the plasmid, there is a sfGFP coding device which contains promoter BBa_J23116 and RBS BBa_B0034. We design primer and do PCR of this sfGFP coding device and ligate it to BBa_B0015 (adding a terminator) that we do double enzyme digestion, EcoRI & XbaI.

Source

sfGFP+B0015

References