Difference between revisions of "Part:BBa K2042002"
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| − | a | + | This is the coding sequence for a Polyhydroxyalkanoate synthase from Pseudomonas sp. The enzyme takes the monomer (D)-lactyl-CoA and creates the PLA homopolymer. This sequence includes four mutations which were found to increase yields of PLA (Yang et al.) |
| + | The sequence is codon optimized for Pseudomonas putida | ||
| + | |||
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Revision as of 16:43, 4 September 2016
Pseudomonas sp. MBEL 6-19 PHA synthase 1
This is the coding sequence for a Polyhydroxyalkanoate synthase from Pseudomonas sp. The enzyme takes the monomer (D)-lactyl-CoA and creates the PLA homopolymer. This sequence includes four mutations which were found to increase yields of PLA (Yang et al.) The sequence is codon optimized for Pseudomonas putida
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1060
Illegal NgoMIV site found at 1339
Illegal AgeI site found at 135
Illegal AgeI site found at 448 - 1000COMPATIBLE WITH RFC[1000]