Difference between revisions of "Part:BBa K2066053:Design"

 
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===Design Notes===
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===Design Notes==
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Made this part to make multiple plasmid transformations more effective.  
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The part was made to put K2066022 and K2066023 on the same plasmid.  
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Revision as of 02:55, 7 September 2016


pTET GFP + TetR on UNS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1004
    Illegal NheI site found at 1027
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 764


=Design Notes

The part was made to put K2066022 and K2066023 on the same plasmid.


Source

This part was derived mostly from iGEM registry parts. It is a combination of K2066022 and K2066023. It uses UNS sequences from Torella et. al 2013 ("Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly")

References