Difference between revisions of "Part:BBa K2151001"
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| − | + | <p>This part consists of p25, a medium constitutive bacterial promoter, contained within pSB1C3. Its intended use is to promote transcription of an inserted gene. p25 was initially identified in a promoter probe survey of the <i>Streptococcus thermophilus</i> genome, alongside weaker promoters p8 and p20.</p> | |
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| + | <p>We used p25 as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of p25 using GFP revealed promoter strength on par with medium Anderson promoters (30.8% as strong as J23100, 43.7% as strong as J23101 130% as strong as J23106).</p> | ||
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Revision as of 20:49, 8 October 2016
p25, moderate constitutive S. thermophilus promoter
This part consists of p25, a medium constitutive bacterial promoter, contained within pSB1C3. Its intended use is to promote transcription of an inserted gene. p25 was initially identified in a promoter probe survey of the Streptococcus thermophilus genome, alongside weaker promoters p8 and p20.
We used p25 as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of p25 using GFP revealed promoter strength on par with medium Anderson promoters (30.8% as strong as J23100, 43.7% as strong as J23101 130% as strong as J23106).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]