Difference between revisions of "Part:BBa K2042008"
RATOVOMANANA (Talk | contribs) |
|||
| Line 3: | Line 3: | ||
<partinfo>BBa_K2042008 short</partinfo> | <partinfo>BBa_K2042008 short</partinfo> | ||
| − | This is the coding sequence of lactate deshydrogenase that can super efficiently use NADPH and NADH as cofactor | + | This is the coding sequence of lactate deshydrogenase that can super efficiently use NADPH and NADH as cofactor. Point mutations on three amino acids (D176S, I177R, F178T) resulted in a modified enzyme designated as D-LDH*. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 18:38, 1 October 2016
Engineered D-Lactate dehydrogenase (D-LDH*)
This is the coding sequence of lactate deshydrogenase that can super efficiently use NADPH and NADH as cofactor. Point mutations on three amino acids (D176S, I177R, F178T) resulted in a modified enzyme designated as D-LDH*.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 630
- 1000COMPATIBLE WITH RFC[1000]