Difference between revisions of "Part:BBa K2180010"

Revision as of 15:40, 23 October 2016

Cadmium Operator - MntR Binding Site

Biobrick based on a previous biobrick made by the iGEM09_Cornell Team (2009-08-10) :
http://partsregistry.org/Part:BBa_K285103:Design
We added two restriction sites: SacII before and PacI after the regulatory region in order to use it in our project. They allow us to replace a regulatory region with another regulatory region. This Region allow MntR to bind to DNA and repress transcription. In the presence of Cadmium transcription is derepressed because of the fixation of cadmium to MntR.

Other metallic ions may bind to MntR.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2180010 short</partinfo>
-We add two restriction sites. SacII before and PacI after the regulatory region in order to use it in our project. They allow us to replace a regulatory region with another regulatory region. This Region allow MntR to bind to DNA and repress transcription. In the presence of Cadmium transcription is derepressed because of the fixation of cadmium to MntR.
+Biobrick based on a previous biobrick made by the iGEM09_Cornell Team (2009-08-10) :
-Other metalic ions may bind to MntR.
+<br>http://partsregistry.org/Part:BBa_K285103:Design
+<br>We added two restriction sites: SacII before and PacI after the regulatory region in order to use it in our project. They allow us to replace a regulatory region with another regulatory region. This Region allow MntR to bind to DNA and repress transcription. In the presence of Cadmium transcription is derepressed because of the fixation of cadmium to MntR.
+*Other metallic ions may bind to MntR.
 <!-- Add more about the biology of this part here