Difference between revisions of "Part:BBa K1983000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Transporter
 
  
 +
===Primers===
  
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Primers used for amplification of the fragment:
 +
PAL-Chis-ex-FW: AACGTCTCCCATGAAGACCCTGTCCCAG
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PAL-Chis-ex-RV: AACTCGAGATGCAGGCTAGGCAGG
 +
 +
Primers used for colony PCR screening:
 +
 +
For pBAD:
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Bad-P1: AGATTAGCGGATCCTACCTGACGC
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Bad-T1: TGTTTTATCAGACCGCTTCTGCGTTC
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 +
For pETDuet-1:
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Up-A1: GGATCTCGACGCTCTCCCT
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Down3: ACCCCTCAAGACCCGTTTAG
  
 
===Source===
 
===Source===

Revision as of 13:02, 21 October 2016


Codon optimized AvPAL with C-terminal 6XHis-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1265
    Illegal XhoI site found at 1702
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1483
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers

Primers used for amplification of the fragment: PAL-Chis-ex-FW: AACGTCTCCCATGAAGACCCTGTCCCAG PAL-Chis-ex-RV: AACTCGAGATGCAGGCTAGGCAGG

Primers used for colony PCR screening:

For pBAD: Bad-P1: AGATTAGCGGATCCTACCTGACGC Bad-T1: TGTTTTATCAGACCGCTTCTGCGTTC

For pETDuet-1: Up-A1: GGATCTCGACGCTCTCCCT Down3: ACCCCTCAAGACCCGTTTAG

Source

E coli

References