Difference between revisions of "Part:BBa K1983000"
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<partinfo>BBa_K1983000 short</partinfo> | <partinfo>BBa_K1983000 short</partinfo> | ||
− | + | This is an improved Stanford-Brown 2013 PAL biobrick part. We have removed the T7 promoter and replaced the N-terminal FLAG-tag with a more common C-terminal 6xHis-tag. The C-terminal tag location was chosen assuming it would less likely affect the stability and folding of the protein. | |
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Revision as of 09:19, 17 October 2016
Codon optimized AvPAL with C-terminal 6XHis-Tag
This is an improved Stanford-Brown 2013 PAL biobrick part. We have removed the T7 promoter and replaced the N-terminal FLAG-tag with a more common C-terminal 6xHis-tag. The C-terminal tag location was chosen assuming it would less likely affect the stability and folding of the protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1265
Illegal XhoI site found at 1702 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1483
- 1000COMPATIBLE WITH RFC[1000]