Difference between revisions of "Part:BBa K2010999"
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PETase is the PET-degrading enzyme first identified in Ideonella sakaiensis. | PETase is the PET-degrading enzyme first identified in Ideonella sakaiensis. | ||
This sequence is the E. coli K12 optimized DNA sequence for PETase. | This sequence is the E. coli K12 optimized DNA sequence for PETase. | ||
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| + | ATGAATTTTCCTCGCGCTTCACGACTGATGCAGGCTGCCGTTCTTGGAGGGCTGATGGCCGTTAGCGCGGCCGCCACCGCTCAGACAAATCCCTACGCCCGCGGTCCGAATCCGACAGCCGCCAGTTTGGAAGCGAGCGCTGGTCCATTCACCGTTCGCTCCTTTACCGTGAGTAGACCGAGCGGTTATGGCGCTGGCACCGTTTACTATCCAACAAATGCTGGGGGTACCGTGGGCGCCATAGCCATAGTTCCCGGGTATACGGCACGGCAGTCATCAATTAAATGGTGGGGACCGCGTCTGGCATCCCACGGTTTCGTAGTAATTACAATTGACACAAATTCCACGTTAGACCAGCCATCAAGTCGGAGTTCGCAACAAATGGCCGCGCTGCGCCAGGTGGCGTCGTTAAACGGTACAAGTAGCAGCCCGATTTACGGAAAGGTCGATACCGCTCGTATGGGTGTTATGGGGTGGAGTATGGGAGGTGGAGGCTCCCTGATCTCTGCTGCTAACAACCCTTCGCTGAAAGCAGCGGCGCCTCAAGCACCATGGGATTCTTCGACAAATTTTAGTTCTGTAACTGTGCCCACGCTGATCTTCGCATGTGAAAACGATAGTATAGCCCCGGTCAACTCTTCAGCACTTCCTATCTATGATTCTATGTCACGCAACGCTAAGCAGTTTCTCGAAATTAATGGTGGCTCACATTCCTGTGCGAATAGCGGCAATTCTAACCAAGCATTAATCGGAAAAAAAGGCGTTGCATGGATGAAACGTTTTATGGACAATGATACTAGGTATTCTACTTTTGCCTGCGAGAACCCGAATAGCACCAGAGTGTCTGATTTTCGTACAGCGAATTGCAGCTGA | ||
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Revision as of 02:59, 17 October 2019
PETase (PET-degrading enzyme, origin I. sakaiensis)
PETase is the PET-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.
ATGAATTTTCCTCGCGCTTCACGACTGATGCAGGCTGCCGTTCTTGGAGGGCTGATGGCCGTTAGCGCGGCCGCCACCGCTCAGACAAATCCCTACGCCCGCGGTCCGAATCCGACAGCCGCCAGTTTGGAAGCGAGCGCTGGTCCATTCACCGTTCGCTCCTTTACCGTGAGTAGACCGAGCGGTTATGGCGCTGGCACCGTTTACTATCCAACAAATGCTGGGGGTACCGTGGGCGCCATAGCCATAGTTCCCGGGTATACGGCACGGCAGTCATCAATTAAATGGTGGGGACCGCGTCTGGCATCCCACGGTTTCGTAGTAATTACAATTGACACAAATTCCACGTTAGACCAGCCATCAAGTCGGAGTTCGCAACAAATGGCCGCGCTGCGCCAGGTGGCGTCGTTAAACGGTACAAGTAGCAGCCCGATTTACGGAAAGGTCGATACCGCTCGTATGGGTGTTATGGGGTGGAGTATGGGAGGTGGAGGCTCCCTGATCTCTGCTGCTAACAACCCTTCGCTGAAAGCAGCGGCGCCTCAAGCACCATGGGATTCTTCGACAAATTTTAGTTCTGTAACTGTGCCCACGCTGATCTTCGCATGTGAAAACGATAGTATAGCCCCGGTCAACTCTTCAGCACTTCCTATCTATGATTCTATGTCACGCAACGCTAAGCAGTTTCTCGAAATTAATGGTGGCTCACATTCCTGTGCGAATAGCGGCAATTCTAACCAAGCATTAATCGGAAAAAAAGGCGTTGCATGGATGAAACGTTTTATGGACAATGATACTAGGTATTCTACTTTTGCCTGCGAGAACCCGAATAGCACCAGAGTGTCTGATTTTCGTACAGCGAATTGCAGCTGA
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 67
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]