Difference between revisions of "Part:BBa J100266"

Revision as of 20:59, 17 June 2016

pJC173g-SD8_AP-neg (Carlson et al. 2014)

In their 2014 paper "[http://www.nature.com/nchembio/journal/v10/n3/full/nchembio.1453.html Negative selection and stringency modulation in phage-assisted continuous evolution]," Carlson et al., designed part J100265 to be used as an accessory plasmid in phage-assisted continuous evolution (PACE). This DNA part comes from a collection of 8 plasmids numbers J100264 - J100271. PACE employs M13 phage and E. coli in a chemostat.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1292
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1292
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1991
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1292
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1292
Illegal NgoMIV site found at 3352
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 981
Illegal BsaI.rc site found at 3060
Illegal SapI site found at 2158
Illegal SapI.rc site found at 790

@@ Line 3: / Line 3: @@
 <partinfo>BBa_J100266 short</partinfo>
-In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100266 to be used as an accessory plasmid in phage-assisted continuous evolution (PACE).
+In their 2014 paper "[http://www.nature.com/nchembio/journal/v10/n3/full/nchembio.1453.html Negative selection and stringency modulation in phage-assisted continuous evolution]," Carlson ''et al.'', designed part J100265 to be used as an accessory plasmid in phage-assisted continuous evolution (PACE). This DNA part comes from a collection of 8 plasmids numbers J100264 - J100271. PACE employs M13 phage and ''E. coli'' in a chemostat.
 <!-- Add more about the biology of this part here