Difference between revisions of "RFC25 "Freiburg standard" parts"
VolkerMorath (Talk | contribs) (→Fluorescent proteins) |
VolkerMorath (Talk | contribs) (→Fluorescent proteins) |
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| Line 13: | Line 13: | ||
|Cerulean | |Cerulean | ||
|<span style="background:Cyan; color:black;"> Cyan </span> | |<span style="background:Cyan; color:black;"> Cyan </span> | ||
| − | |BBa_I757009 | + | |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_I757009'>BBa_I757009</A></HTML> |
|433 | |433 | ||
|475 | |475 | ||
| Line 27: | Line 27: | ||
|GFP | |GFP | ||
|<span style="background:Lime; color:black;"> Green </span> | |<span style="background:Lime; color:black;"> Green </span> | ||
| − | |BBa_K863120 | + | |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K863120'>BBa_K863120</A></HTML> |
|484 | |484 | ||
|507 | |507 | ||
| Line 34: | Line 34: | ||
|GFP | |GFP | ||
|<span style="background:Lime; color:black;"> Green </span> | |<span style="background:Lime; color:black;"> Green </span> | ||
| − | |BBa_K823039 | + | |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K823039'>BBa_K823039</A></HTML> |
|484 | |484 | ||
|507 | |507 | ||
| Line 48: | Line 48: | ||
|Dronpa (photoactivatable) | |Dronpa (photoactivatable) | ||
|<span style="background:Lime; color:black;"> Green </span> | |<span style="background:Lime; color:black;"> Green </span> | ||
| − | |BBa_K1680006 | + | |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1680006'>BBa_K1680006</A></HTML> |
|503 | |503 | ||
|518 | |518 | ||
| Line 55: | Line 55: | ||
|mVenus | |mVenus | ||
|<span style="background:Yellow; color:black;"> Yellow </span> | |<span style="background:Yellow; color:black;"> Yellow </span> | ||
| − | |BBa_I757008 | + | |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_I757008'>BBa_I757008</A></HTML> |
|515 | |515 | ||
|528 | |528 | ||
| Line 76: | Line 76: | ||
|mRFP | |mRFP | ||
|<span style="background:red; color:black;"> Red </span> | |<span style="background:red; color:black;"> Red </span> | ||
| − | |BBa_K1351021 | + | |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1351021'>BBa_K1351021</A></HTML> |
|584 | |584 | ||
|607 | |607 | ||
| Line 90: | Line 90: | ||
|mKate2 | |mKate2 | ||
|<span style="background:red; color:black;"> Red </span> | |<span style="background:red; color:black;"> Red </span> | ||
| − | |BBa_K823029 | + | |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K823029'>BBa_K823029</A></HTML> |
|588 | |588 | ||
|633 | |633 | ||
Revision as of 00:20, 15 May 2016
Contents
Reptorter proteins
Fluorescent proteins
| Fluorescent proteins | |||||
|---|---|---|---|---|---|
| Protein | Color | BioBrick | Excitation [nm] | Emission [nm] | Quantum Yield |
| Cerulean | Cyan | BBa_I757009 | 433 | 475 | 0.62 |
| GFP | Green | BBa_K648013 | 484 | 507 | 0.60 |
| GFP | Green | BBa_K863120 | 484 | 507 | 0.60 |
| GFP | Green | BBa_K823039 | 484 | 507 | 0.60 |
| sfGFP | Green | BBa_K1321337 | 485 | 510 | 0.65 |
| Dronpa (photoactivatable) | Green | BBa_K1680006 | 503 | 518 | 0.85 |
| mVenus | Yellow | BBa_I757008 | 515 | 528 | 0.57 |
| sYFP2 | Yellow | BBa_K1159301 | 514 | 527 | 0.61 |
| eCFP | Cyan | BBa_K1159302 | 439 | 476 | 0.40 |
| mRFP | Red | BBa_K1351021 | 584 | 607 | 0.25 |
| mRFP | Red | BBa_K1615089 | 584 | 607 | 0.25 |
| mKate2 | Red | BBa_K823029 | 588 | 633 | |
Values for the fluorescent proteins were taken from http://www.microscopyu.com/articles/livecellimaging/fpintro.html.
Luciferases
| Luciferases | |||
|---|---|---|---|
| Protein | BioBrick | Size [kDa] | |
| Firefly luciferase | BBa_K525999 | 61 | |
| Renilla luciferase | 36 | ||
| NanoLuc luciferase | BBa_K1159001 | 19 | |
| Gaussia luciferase | |||
Transmembrane Domains
Linker domains
| Linker | |||
|---|---|---|---|
| Protein | Sequence | BioBrick | Length [residues] |
| Short Linker | (Gly-Gly-Ser-Gly) | BBa_K243004 | 4 |
| Middle Linker | 2x (Gly-Gly-Ser-Gly) | BBa_K243005 | 8 |
| Long Linker | 3x (Gly-Gly-Ser-Gly) | BBa_K243006 | 12 |
Catalytic domains
| Fluorescent proteins | |||||
|---|---|---|---|---|---|
| Erythromycin Esterase Type II | BBa_K1159000 | ||||
| Laccase | BBa_K1159002 | ||||
| Laccase TVEL5 | BBa_K1615067 | ||||
| TEV Protease | BBa_K1159100 | ||||
| Thermonuclease | BBa_K1159105 | ||||
| Heroin Esterase | BBa_K1615045 | ||||
| CRE recombinase | BBa_K1680007 | ||||
| Monoamine Oxidase A | BBa_K1615022 | ||||
| Endo-β-Galactosidase | BBa_K1483001 | ||||
| α-Galactosidase | BBa_K1483002 | ||||
| Acetylgalactosamindase | BBa_K1483000 | ||||
| 2-oxoglutarate oxygenase/decarboxylase | BBa_K1065000 | ||||
Transcription Regulators
| Fluorescent proteins | |||||
|---|---|---|---|---|---|
| VP16 | BBa_K1150001 | ||||
Split proteins
| Fluorescent proteins | |||||
|---|---|---|---|---|---|
| TEV Protease | BBa_K1159101 | BBa_K1159102 | |||
| SpyCatcher/SpyTag | BBa_K1159200 | BBa_K1159201 | |||
Binding proteins
| Fluorescent proteins | |||||
|---|---|---|---|---|---|
| Fluorescein binding Anticalin | BBa_K1159003 | ||||
| Digoxigenin binding Anticalin | BBa_K243003 | ||||
| HER2 binding DARPin | BBa_K404302 | ||||
| HER2 binding Affibody | BBa_K404302 | ||||
| Nickel Binding Protein | BBa_K1321009 | ||||
| Cellulose binding Domain | BBa_K863110 | ||||
Affinity tags
| Affinity tags | |||||
|---|---|---|---|---|---|
| HA tag | BBa_K1150016 | ||||
| His6-tag | BBa_I757013 | ||||
| SpyTag | BBa_K1159020 | ||||
| Strep-tag II as N-part | BBa_K549018 | ||||
| Strep-tag II | BBa_J18916 | ||||
| cMyc-tag | BBa_K823036 | ||||
| 3x FLAG tag | BBa_K823034 | ||||