Difference between revisions of "RFC25 "Freiburg standard" parts"

(→‎Luciferases)
(→‎Reptorter proteins)
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==Catalytic domains==
 
==Catalytic domains==
−
Erythromycin Esterase Type II  
+
{| class="wikitable"
−
BBa_K1159000
+
!colspan="6"|Fluorescent proteins
−
 
+
|-
−
Laccase
+
|Erythromycin Esterase Type II  
−
BBa_K1159002
+
|BBa_K1159000
−
 
+
|-
−
Laccase TVEL5
+
|Laccase
−
BBa_K1615067
+
|BBa_K1159002
−
 
+
|-
−
TEV Protease
+
|Laccase TVEL5
−
BBa_K1159100
+
|BBa_K1615067
−
 
+
|-
−
Thermonuclease
+
|TEV Protease
−
BBa_K1159105
+
|BBa_K1159100
−
 
+
|-
−
Heroin Esterase
+
|Thermonuclease
−
BBa_K1615045
+
|BBa_K1159105
−
 
+
|-
−
CRE recombinase
+
|Heroin Esterase
−
BBa_K1680007
+
|BBa_K1615045
−
 
+
|-
−
Monoamine Oxidase A  
+
|CRE recombinase
−
BBa_K1615022
+
|BBa_K1680007
−
 
+
|-
−
Endo-β-Galactosidase
+
|Monoamine Oxidase A  
−
BBa_K1483001
+
|BBa_K1615022
−
 
+
|-
−
α-Galactosidase
+
|Endo-β-Galactosidase
−
BBa_K1483002
+
|BBa_K1483001
−
 
+
|-
−
Acetylgalactosamindase
+
|α-Galactosidase
−
BBa_K1483000
+
|BBa_K1483002
−
 
+
|-
−
2-oxoglutarate oxygenase/decarboxylase
+
|Acetylgalactosamindase
−
BBa_K1065000
+
|BBa_K1483000
−
 
+
|-
 +
|2-oxoglutarate oxygenase/decarboxylase
 +
|BBa_K1065000
 +
|-
 +
|}
 
==Transcription Regulators==
 
==Transcription Regulators==
−
VP16
+
|VP16
−
BBa_K1150001
+
|BBa_K1150001
−
 
+
|-
 +
|}
 
==Split proteins==
 
==Split proteins==
−
TEV Protease
+
|TEV Protease
−
BBa_K1159101
+
|BBa_K1159101
−
BBa_K1159102
+
|BBa_K1159102
−
 
+
|-
−
SpyCatcher/SpyTag
+
|SpyCatcher/SpyTag
−
BBa_K1159200
+
|BBa_K1159200
−
BBa_K1159201
+
|BBa_K1159201
−
 
+
|-
 +
|}
 
==Binding proteins==
 
==Binding proteins==
−
Fluorescein binding Anticalin  
+
{| class="wikitable"
−
BBa_K1159003
+
!colspan="6"|Fluorescent proteins
−
 
+
|-
−
Digoxigenin binding Anticalin
+
|Fluorescein binding Anticalin  
−
BBa_K243003
+
|BBa_K1159003
−
 
+
|-
−
HER2 binding DARPin
+
|Digoxigenin binding Anticalin
−
BBa_K404302
+
|BBa_K243003
−
 
+
|-
−
HER2 binding Affibody
+
|HER2 binding DARPin
−
BBa_K404302
+
|BBa_K404302
−
 
+
|-
−
Nickel Binding Protein
+
|HER2 binding Affibody
−
BBa_K1321009
+
|BBa_K404302
−
 
+
|-
−
Cellulose binding Domain
+
|Nickel Binding Protein
−
BBa_K863110
+
|BBa_K1321009
−
 
+
|-
 +
|Cellulose binding Domain
 +
|BBa_K863110
 +
|}
 
==Protein tags==
 
==Protein tags==
−
''Strep''-tag II an N-part
+
{| class="wikitable"
−
BBa_K549018
+
!colspan="6"|Fluorescent proteins
−
 
+
|-
−
HA tag
+
|''Strep''-tag II an N-part
−
BBa_K1150016
+
|BBa_K549018
−
 
+
|-
−
His<sub>6</sub>-tag
+
|HA tag
−
BBa_I757013
+
|BBa_K1150016
−
 
+
|-
−
SpyTag
+
|His<sub>6</sub>-tag
−
BBa_K1159020
+
|BBa_I757013
−
 
+
|-
−
''Strep''-tag II
+
|SpyTag
−
BBa_J18916
+
|BBa_K1159020
−
 
+
|-
−
cMyc-tag
+
|''Strep''-tag II
−
BBa_K823036
+
|BBa_J18916
−
 
+
|-
−
3x FLAG tag
+
|cMyc-tag
−
BBa_K823034
+
|BBa_K823036
−
 
+
|-
 +
|3x FLAG tag
 +
|BBa_K823034
 +
|}
 
<groupparts>iGEM07 Freiburg</groupparts>
 
<groupparts>iGEM07 Freiburg</groupparts>

Revision as of 00:10, 15 May 2016

Reptorter proteins

Fluorescent proteins

Fluorescent proteins
Protein Color BioBrick Excitation [nm] Emission [nm] Quantum Yield
Cerulean BBa_I757009
GFP Green BBa_K648013 484 507 0.60
GFP BBa_K863120
GFP BBa_K823039
sfGFP Green BBa_K1321337 485 510 0.65
Dronpa (photoactivatable) Green BBa_K1680006 503 518
mVenus BBa_I757008
sYFP2 Yellow BBa_K1159301 514 527 0.61
eCFP Cyan BBa_K1159302 439 476 0.40
mRFP BBa_K1351021 584 607
mRFP Red BBa_K1615089 584 607 0.25
mKate2 BBa_K823029 588 633

Values for the fluorescent proteins were taken from http://www.microscopyu.com/articles/livecellimaging/fpintro.html.

Luciferases

Luciferases
Protein BioBrick Size [kDa]
Firefly luciferase BBa_K525999 61
Renilla luciferase 36
NanoLuc luciferase BBa_K1159001 19
Gaussia luciferase

Transmembrane Domains

Linker domains

Linker
Protein Sequence BioBrick Length [residues]
Short Linker (Gly-Gly-Ser-Gly) BBa_K243004 4
Middle Linker 2x (Gly-Gly-Ser-Gly) BBa_K243005 8
Long Linker 3x (Gly-Gly-Ser-Gly) BBa_K243006 12

Catalytic domains

Fluorescent proteins
Erythromycin Esterase Type II BBa_K1159000
Laccase BBa_K1159002
Laccase TVEL5 BBa_K1615067
TEV Protease BBa_K1159100
Thermonuclease BBa_K1159105
Heroin Esterase BBa_K1615045
CRE recombinase BBa_K1680007
Monoamine Oxidase A BBa_K1615022
Endo-β-Galactosidase BBa_K1483001
α-Galactosidase BBa_K1483002
Acetylgalactosamindase BBa_K1483000
2-oxoglutarate oxygenase/decarboxylase BBa_K1065000

Transcription Regulators

|VP16 |BBa_K1150001 |- |}

Split proteins

|TEV Protease |BBa_K1159101 |BBa_K1159102 |- |SpyCatcher/SpyTag |BBa_K1159200 |BBa_K1159201 |- |}

Binding proteins

Fluorescent proteins
Fluorescein binding Anticalin BBa_K1159003
Digoxigenin binding Anticalin BBa_K243003
HER2 binding DARPin BBa_K404302
HER2 binding Affibody BBa_K404302
Nickel Binding Protein BBa_K1321009
Cellulose binding Domain BBa_K863110

Protein tags

Fluorescent proteins
Strep-tag II an N-part BBa_K549018
HA tag BBa_K1150016
His6-tag BBa_I757013
SpyTag BBa_K1159020
Strep-tag II BBa_J18916
cMyc-tag BBa_K823036
3x FLAG tag BBa_K823034
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