Difference between revisions of "RFC25 "Freiburg standard" parts"

(→‎Catalytic domains)
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|Dronpa (photoactivatable)
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|<span style="background:Lime; color:white;"> Green </span>
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|BBa_K1680006
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|518
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|sYFP2
 
|sYFP2

Revision as of 22:57, 14 May 2016

Reptorter proteins

Fluorescent proteins

Fluorescent proteins
Protein Color BioBrick Excitation [nm] Emission [nm] Quantum Yield
GFP Green BBa_K648013 484 507 0.60
sfGFP Green BBa_K1321337 485 510 0.65
Dronpa (photoactivatable) Green BBa_K1680006 503 518
sYFP2 Yellow BBa_K1159301 514 527 0.61
eCFP Cyan BBa_K1159302 439 476 0.40
mRFP Red BBa_K1615089 584 607 0.25

Values for the fluorescent proteins were taken from http://www.microscopyu.com/articles/livecellimaging/fpintro.html.

Luciferases

Luciferases
Protein BioBrick Size [kDa]
Firefly luciferase 61
Renilla luciferase 36
NanoLuc luciferase BBa_K1159001 19
Gaussia luciferase

Transmembrane Domains

Linker domains

Linker
Protein Sequence BioBrick Length [residues]
Short Linker (Gly-Gly-Ser-Gly) BBa_K243004 4
Middle Linker 2x (Gly-Gly-Ser-Gly) BBa_K243005 8
Long Linker 3x (Gly-Gly-Ser-Gly) BBa_K243006 12

Catalytic domains

Erythromycin Esterase Type II BBa_K1159000

Laccase BBa_K1159002

Laccase TVEL5 BBa_K1615067

TEV Protease BBa_K1159100

Thermonuclease BBa_K1159105

Heroin Esterase BBa_K1615045

CRE recombinase BBa_K1680007

Split proteins

TEV Protease BBa_K1159101 BBa_K1159102

SpyCatcher/SpyTag BBa_K1159200 BBa_K1159201

Binding proteins

Fluorescein-Binding Anticalin BBa_K1159003

Protein tags

Strep-tag II an N-part BBa_K549018

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