Difference between revisions of "RFC25 "Freiburg standard" parts"

(→‎Reptorter proteins)
(→‎Fluorescent proteins)
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==Fluorescent proteins==
 
==Fluorescent proteins==
 
{| class="wikitable"
 
{| class="wikitable"
−
!colspan="5"|Fluorescent proteins
+
!colspan="6"|Fluorescent proteins
 
|-
 
|-
 
|Protein
 
|Protein
 
|Color
 
|Color
 
|BioBrick
 
|BioBrick
−
|Excitation
+
|Excitation [nm]
−
|Emission
+
|Emission [nm]
−
 
+
|Quantum Yield
 
|-
 
|-
 
|GFP
 
|GFP
 
|<span style="background:Lime; color:white;"> Green </span>
 
|<span style="background:Lime; color:white;"> Green </span>
 
|[[BBa_K648013]]
 
|[[BBa_K648013]]
−
|
+
|484
−
|
+
|507
 +
|0.60
 
|-
 
|-
 
|sfGFP
 
|sfGFP
 
|<span style="background:Lime; color:white;"> Green </span>
 
|<span style="background:Lime; color:white;"> Green </span>
 
|[[BBa_K1321337]]
 
|[[BBa_K1321337]]
−
|
+
|485
−
|
+
|510
 +
|0.65
 
|-
 
|-
−
|SYFP2
+
|sYFP2
 
|<span style="background:Yellow; color:black;"> Yellow </span>
 
|<span style="background:Yellow; color:black;"> Yellow </span>
 
|[[BBa_K1159301]]
 
|[[BBa_K1159301]]
−
|
+
|514
−
|
+
|527
 +
|0.61
 
|-
 
|-
 
|eCFP
 
|eCFP
 
|<span style="background:DeepPink; color:black;"> Cyan </span>
 
|<span style="background:DeepPink; color:black;"> Cyan </span>
 
|[[BBa_K1159302]]
 
|[[BBa_K1159302]]
−
|
+
|439
−
|
+
|476
 +
|0.40
 
|-
 
|-
 
|mRFP
 
|mRFP
 
|<span style="background:red; color:black;"> Red </span>
 
|<span style="background:red; color:black;"> Red </span>
 
|[[BBa_K1615089]]
 
|[[BBa_K1615089]]
−
|
+
|584
−
|
+
|607
−
 
+
|0.25
 
|}
 
|}
  

Revision as of 21:59, 14 May 2016

Reptorter proteins

Fluorescent proteins

Fluorescent proteins
Protein Color BioBrick Excitation [nm] Emission [nm] Quantum Yield
GFP Green BBa_K648013 484 507 0.60
sfGFP Green BBa_K1321337 485 510 0.65
sYFP2 Yellow BBa_K1159301 514 527 0.61
eCFP Cyan BBa_K1159302 439 476 0.40
mRFP Red BBa_K1615089 584 607 0.25

Luciferases

Fluorescent proteins
Protein BioBrick Size [kDa]
Firefly luciferase 61
Renilla luciferase 36
NanoLuc luciferase BBa_K1159001 19
Gaussia luciferase