Difference between revisions of "Part:BBa J119392"
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Oligonucleotides that encoded Bicistronic RBSs of the form NNNGANNN were cloned with BsaI Golden Gate Assembly into rClone Red ([https://parts.igem.org/Part:BBa_J119384 BBa_J119384]). to produce a library of 4096 possibilities. Clones were picked from the library and sequenced. The RBS strength of each RBS clone was measured with fluorometry and is expressed as a percentage of the strongest clone found in the project, which is a bicistronic C dog RBS. Below is a table of the RBS sequences and strengths, and a consensus sequence that takes into account every base at every position and the relative RBS strengths. | Oligonucleotides that encoded Bicistronic RBSs of the form NNNGANNN were cloned with BsaI Golden Gate Assembly into rClone Red ([https://parts.igem.org/Part:BBa_J119384 BBa_J119384]). to produce a library of 4096 possibilities. Clones were picked from the library and sequenced. The RBS strength of each RBS clone was measured with fluorometry and is expressed as a percentage of the strongest clone found in the project, which is a bicistronic C dog RBS. Below is a table of the RBS sequences and strengths, and a consensus sequence that takes into account every base at every position and the relative RBS strengths. | ||
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Latest revision as of 16:33, 29 April 2016
Collection of Bicistronic RBSs from N6 rClone Red Library
Oligonucleotides that encoded Bicistronic RBSs of the form NNNGANNN were cloned with BsaI Golden Gate Assembly into rClone Red (BBa_J119384). to produce a library of 4096 possibilities. Clones were picked from the library and sequenced. The RBS strength of each RBS clone was measured with fluorometry and is expressed as a percentage of the strongest clone found in the project, which is a bicistronic C dog RBS. Below is a table of the RBS sequences and strengths, and a consensus sequence that takes into account every base at every position and the relative RBS strengths.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]