Difference between revisions of "Part:BBa J119391"
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Oligonucleotides that encoded RBSs of the form NNNNNNNN were cloned with BsaI Golden Gate Assembly into rClone Red ([https://parts.igem.org/Part:BBa_J119384 BBa_J119384]). to produce a library of 65,536 possibilities. Clones were picked from the library and sequenced. The RBS strength of each RBS clone was measured with fluorometry and is expressed as a percentage of the strongest clone found in the project, which is a bicistronic C dog RBS. Below is a table of the RBS sequences and strengths, and a consensus sequence that takes into account every base at every position and the relative RBS strengths. | Oligonucleotides that encoded RBSs of the form NNNNNNNN were cloned with BsaI Golden Gate Assembly into rClone Red ([https://parts.igem.org/Part:BBa_J119384 BBa_J119384]). to produce a library of 65,536 possibilities. Clones were picked from the library and sequenced. The RBS strength of each RBS clone was measured with fluorometry and is expressed as a percentage of the strongest clone found in the project, which is a bicistronic C dog RBS. Below is a table of the RBS sequences and strengths, and a consensus sequence that takes into account every base at every position and the relative RBS strengths. | ||
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Latest revision as of 16:24, 29 April 2016
Collection of RBSs from N8 rClone Red Library
Oligonucleotides that encoded RBSs of the form NNNNNNNN were cloned with BsaI Golden Gate Assembly into rClone Red (BBa_J119384). to produce a library of 65,536 possibilities. Clones were picked from the library and sequenced. The RBS strength of each RBS clone was measured with fluorometry and is expressed as a percentage of the strongest clone found in the project, which is a bicistronic C dog RBS. Below is a table of the RBS sequences and strengths, and a consensus sequence that takes into account every base at every position and the relative RBS strengths.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]