Difference between revisions of "Part:BBa J119390"

Latest revision as of 16:21, 29 April 2016

Collection of RBSs from N6 rClone Red Library

Oligonucleotides that encoded RBSs of the form NNNGANNN were cloned with BsaI Golden Gate Assembly into rClone Red (BBa_J119384). to produce a library of 4096 possibilities. Clones were picked from the library and sequenced. The RBS strength of each RBS clone was measured with fluorometry and is expressed as a percentage of the strongest clone found in the project, which is a bicistronic C dog RBS. Below is a table of the RBS sequences and strengths, and a consensus sequence that takes into account every base at every position and the relative RBS strengths.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_J119390 short</partinfo>
-Oligonucleotides that encoded RBSs of the form NNNGANNN were cloned with BsaI Golden Gate Assembly into rClone Red [https://parts.igem.org/Part:BBa_J119384 rClone Red]. to produce a library of 4096 possibilities. Clones were picked from the library and sequenced. The RBS strength  of each RBS clone was measured with fluorometry and is expressed as a percentage of the strongest clone found in the project, which is a bicistronic C dog RBS. Below is a table of the RBS sequences and strengths, and a consensus sequence that takes into account every base at every position and the relative RBS strengths.
+Oligonucleotides that encoded RBSs of the form NNNGANNN were cloned with BsaI Golden Gate Assembly into  rClone Red ([https://parts.igem.org/Part:BBa_J119384 BBa_J119384]). to produce a library of 4096 possibilities. Clones were picked from the library and sequenced. The RBS strength  of each RBS clone was measured with fluorometry and is expressed as a percentage of the strongest clone found in the project, which is a bicistronic C dog RBS. Below is a table of the RBS sequences and strengths, and a consensus sequence that takes into account every base at every position and the relative RBS strengths.
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