Difference between revisions of "Repository:iGEM 2007 DNA distribution"

Revision as of 20:23, 25 May 2007

From Mid-April to Early May, the staff at the Registry prepared the Kit of Parts that will be sent to the 2007 iGEM teams.

Here is some useful information about the steps taken in that process.

Quality Control

Antibiotic resistance:

We tested the antibiotic resistance of each part sent out in the Kit of Parts by growing it in broth containing each of our 4 antibiotics. The test was done on a 384-deepwell plate filled with EZ Rich Defined Medium which contained cresol red indicator dye and the appropriate antibiotic. Each quadrant of the test plate contains one of the four antibiotics into which the part is inoculated (see layout below). After allowing the cultures to grow for 18 hrs at 37 C, images from each of the plates are collected. Since the broth contains cresol red indicator that changes color from red to yellow as the pH of the medium decreases, you can tell whether the part grew by noting which of the wells turned yellow (as the cells grow, the medium becomes more acidic).

384-deepwell test plate antibiotic quadrant layout:

Results for each source plate:

Kit Plate Layout

Each 384-well kit plate is a composition of 4 96-well source plates. Below is a representation of which source plates went into which kit plate and in what order.

Kit Plate 1

Kit Plate 2

Kit Plate 3

Kit Plate 4