Difference between revisions of "Part:BBa M36580:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 11: Line 11:
 
   
 
   
 
Sequence before RBS: CAGGATTACA
 
Sequence before RBS: CAGGATTACA
 +
 
Sequence after RBS: TAACCTATCG
 
Sequence after RBS: TAACCTATCG
  

Revision as of 23:36, 23 October 2015


LasR/PAI complex sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added preceding spacing to qsc102 promoter palindromic binding sequence (included in qsc102 part), as well as spacing flanking the RBS in order to optimize binding. The added spacing sequences are detailed below.

Sequence before RBS: CAGGATTACA

Sequence after RBS: TAACCTATCG

Source

qsc102 promoter: Boxed region from Figure 1 of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/ Whiteley spacing: Region following boxed region to bolded A of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/ BBa_0030 strong RBS: partsregistry.org GFP coding sequence: BIOE44 pComet DNA sequence

References