Difference between revisions of "Part:BBa M36242:Design"
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===Source=== | ===Source=== | ||
− | + | Mature EP-B2 DNA sequence and optimized E.Coli his-tag from "Computational Design of an α-Gliadin Peptidase" . YebF secretion system, RBS, terminator and rhamnose promoter from Gene Designer 2.0 | |
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===References=== | ===References=== |
Latest revision as of 23:15, 22 October 2015
Mature EP-B2 YepF Secretion Device
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 262
Illegal NheI site found at 285 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 414
Illegal BglII site found at 1185 - 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 853
Illegal AgeI site found at 937
Illegal AgeI site found at 1276 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1604
Design Notes
Testing whether mature EP-B2 protein is folded properly and is then successfully secreted versus YebF.
Source
Mature EP-B2 DNA sequence and optimized E.Coli his-tag from "Computational Design of an α-Gliadin Peptidase" . YebF secretion system, RBS, terminator and rhamnose promoter from Gene Designer 2.0